Detection of both vanA & vanB genes in vanA phenotypes of Enterococci by Taq Man RT-PCR
نویسندگان
چکیده
Twenty seven isolates of vancomycin resistant Enterococci based on the disk diffusion and E- test have been screened; being found eight (0.3%) clinical isolates of vanA & vanB through Taq Man Real Time PCR assay. This study shows the presence of both vanA & vanB genotypes in vanA phenotypes clinical isolates in the three hospitals in Iran.
منابع مشابه
Evaluation of drug sensitivity of Entrococcus faecium and Entrococcus faecalis Strains and Detection of VanA/B Genes in Vancomycin Resistance Isolated by PCR Method
Enterococci, as the major cause of nosocomial infections, have been shown to have an increase in drug resistance during the past two decades. TheEntrococcalemergence of Vancomycin-resistant is very important nowadays. The purpose of this study was to determine the drug sensitivity of Enterococcus faecalis and Enterococcus faecium species against Vancomycin, Tetracycline, Gentamicin, Erythromyci...
متن کاملتشخیص ژن‌های vanA, vanB, vanC با روش Multiplex-PCR در ایزوله‌های انتروکوکوس فکالیس و انتروکوکوس فسیوم در بیماران بستری
Background & Aims : After Vancomycin resistant Enterococci (VRE) was first detected in the mid 1980s, VRE spread rapidly worldwide and became one of the most serious problems in many hospitals specially in hospitalized patients. Among different enterococcal species Enterococcus feacalis and Enterococus faecium are the leading causes of life threatening infections. VanA/B/C genes are respons...
متن کاملDevelopment and evaluation of a Quadruplex Taq Man real-time PCR assay for simultaneous detection of clinical isolates of Enterococcus faecalis, Enterococcus faecium and their vanA and vanB genotypes
BACKGROUND AND OBJECTIVES We developed and evaluated the utility of a quadruplex Taqman real-time PCR assay that allows simultaneous identification of vancomycin-resistant genotypes and clinically relevant enterococci. MATERIALS AND METHODS The specificity of the assay was tested using reference strains of vancomycin-resistant and susceptible enterococci. In total, 193 clinical isolates were ...
متن کاملMultiplex PCR detection of vanA, vanB, vanC-1, and vanC-2/3 genes in enterococci.
Vancomycin-resistant enterococci (VRE) are increasingly isolated from clinical specimens. One hundred clinical isolates of enterococci (E. casseliflavus/E. flavescens [n = 10], E. faecalis [n = 34], E. faecium [n = 43], E. avium [n = 1], E. gallinarum [n = 11], and E. raffinosus [n = 1]) were examined for the presence of vanA, vanB, vanC-1, and vanC-2/3 genes by a single multiplex PCR performed...
متن کاملبررسی خصوصیات فنوتیپی و ژنوتیپی سویه های انتروکوکوس مقاوم به ونکومایسین جدا شده از نمونه های بالینی بیماران شهرکرد
Background and purpose: Enterococci are important agents of endocarditis and urinary tract infections. They acquire resistance against antimicrobial agents which causes difficulty in their treatment. This study aimed to determine the antimicrobial susceptibility of strains resistant to vancomycin and identification of resistance genes vanA and vanB Enterococcus strains isolated from teaching ho...
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عنوان ژورنال:
دوره 46 شماره
صفحات -
تاریخ انتشار 2015